产品参数
在高能量需求的组织（如心脏）中，多达50%至70%的能量来自脂肪酸β-氧化。脂肪酸β-氧化将多种脂肪酸转化为乙酰辅酶A（acetyl-CoA），同时在细胞线粒体中产生FADH?和NADH。这一严格的有氧氧化途径包括四个不同的步骤：酰基辅酶A脱氢酶催化的酰基辅酶A脱氢作用、烯酰辅酶A水合酶催化的水合作用、3-羟基酰基辅酶A脱氢酶催化的脱氢作用以及硫解酶催化的硫解作用。β-氧化系统的重要性体现在脂肪酸氧化障碍（Fatty Acid Oxidation Disorders, FAODs）这一类遗传性疾病的存在上，这些疾病是脂肪酸运输和氧化功能缺陷的异质性群体。因此，测量脂肪酸氧化（FAO）活性可以为许多疾病的病理生理和代谢基础提供宝贵的见解。
这种非放射性脂肪酸氧化（FAO）检测方法基于长链的棕榈酰辅酶A（palmitoyl-CoA）、中链的辛酰辅酶A（octanoyl-CoA）或短链的丁酰辅酶A（butyryl-CoA）的氧化。NADH的生成与四氮杂卓盐（INT）的还原偶联，生成红色的甲臜（formazan）。红色甲臜的强度与脂肪酸氧化活性的增加成正比。请根据您的需求选择E-141L（棕榈酰辅酶A）、E-141M（辛酰辅酶A）或E-141S（丁酰辅酶A）。E-141L、E-141M和E-141S分别用于评估细胞/组织对长链、中链和短链脂肪酸的氧化能力。
E-141L Kit Components:
FAO Assay Solution: 5 ml, store at -80°C (for 100 wells)
10x FAO Substrate (L): 0.5 ml, store at -80ºC
10x Sample Buffer: 25 ml, store at 4ºC
E-141M Kit Components:
FAO Assay Solution: 5 ml, store at -80°C (for 100 wells)
20x FAO Substrate (M): 0.25 ml, store at -80ºC
10x Sample Buffer: 25 ml, store at 4ºC
E-141S Kit Components:
FAO Assay Solution: 5 ml, store at -80°C (for 100 wells)
25x FAO Substrate (S): 0.2 ml, store at -80ºC
10x Sample Buffer: 25 ml, store at 4ºC
SDS:
DMSO, INT, Tris
Related Kits:
HK Assay, L-Lactate Assay, ATP Assay
Citation:
Kwong et al
Metabolic role of fatty acid binding protein 7 in mediating triple-negative breast cancer cell death via PPAR-alpha signaling
J. Lipid Res. 60:1807, 2019
Cheng et al
Targeting DGAT1 ameliorates glioblastoma by increasing fat catabolism and oxidative stress
Cell Metabolism 32, 229-242, 2020
Chen et al
HNF4 regulates fatty acid oxidation and is required for renewal of intestinal stem cells in mice
Gastroenterology 158(4):985-999, 2020
Veeriah et al
Long-term oral administration of an HNF4α agonist prevents weight gain and hepatic steatosis by promoting increased mitochondrial mass and function
Cell Death & Disease 13(1):89, 2022
Xu et al
Hepatic PRMT1 ameliorates diet-induced hepatic steatosis via induction of PGC1α
Theranostics 12(6):2502-2518, 2022
Huang et al
PERM1 regulates genes involved in fatty acid metabolism in the heart by interacting with PPARα and PGC?1α
Scientific Reports 12:14576, 2022
Sabatini et al.
The proneural transcription factor Atoh1 promotes odontogenic differentiation in human dental pulp stem cells (DPSCs)
BMC Mol Cell Biology 26:1-10, 2025