Description:PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Golimumab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Golimumab will be captured by immobilized Golimumab. After washing away any unbound substances, a biotin-labeled Golimumab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Golimumab bound in the initial step. The color development is stopped and the intensity of the color is measured. Stability and Storage:When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃. Detection method:Colorimetric Sample type:Plasma, Serum Assay type:Quantitative Sensitivity:85.1 ng/mL Range:156.25 - 10,000 ng/mL Recovery:80-120% Background: Shipping:2-8 ℃ Specifications:Golimumab Note:For Research Use Only.