产品参数
γ-氨基丁酸转氨酶（GABAT或ABAT）是一种依赖磷酸吡哆醛的酶，参与抑制性神经递质GABA的降解过程。该酶能催化GABA与α-酮戊二酸转化为谷氨酸和琥珀酸半醛。中枢神经系统中GABA能抑制信号的精确平衡对调节L-谷氨酸介导的兴奋性通路至关重要，因此以GABA系统为靶点的治疗可广泛应用于多种神经及精神疾病。由于抑制GABAT能提升脑内GABA浓度，该酶已成为癫痫治疗的重要靶点。除脊椎动物外，GABAT在微生物、植物和无脊椎动物等多种生物体中均有发现，其活性不仅存在于脑组织，在肝组织中也有体现。美国国立卫生研究院罕见病办公室将GABAT缺乏症列为"罕见病"。
本GABAT检测试剂盒基于连续转氨反应与谷氨酸脱氢酶偶联反应，通过INT还原生成INT-甲臜（492nm处摩尔消光系数为18/mM-cm），可灵敏检测粗提组织样本中的GABAT酶活性。若储存和处理得当，试剂溶液可稳定保存数年。
试剂盒组分：
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GABAT底物：
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2ml
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-80°C保存
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TA检测溶液
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5ml（50次检测）
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-80°C保存
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10×细胞裂解液
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25ml
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4°C保存
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含SDS、TX-100、DMSO、INT、Tris等成分
相关试剂盒：
AST检测试剂盒、ALT检测试剂盒
Product Description:
Gamma-aminobutyrate aminotransferase (GABAT or ABAT) is a pyridoxal phosphate-dependent enzyme involved in the degradation of the inhibitory neurotransmitter GABA. GABAT converts GABA and alpha-ketoglutarate to glutamate and succinic semialdehyde. The fine balance of the GABAergic inhibitory tone in the CNS is critical for countering the excitatory pathway mediated by L-glutamate. Thus, therapeutic targeting of the GABA system covers a broad spectrum of neurological and psychiatric disorders, and GABAT is a validated target for epilepsy therapy because inhibition of the enzyme raises GABA concentrations in the brain. GABAT has been found in a wide variety of species (microorganisms, plants and invertebrates) in addition to vertebrates. GABAT activity is also demonstrable in hepatic tissue in addition to brain. GABAT deficiency is listed as a “rare disease" by the Office of Rare Disease of the NIH. The GABAT Assay Kit is based on sequential transamination reaction and glutamate dehydrogenase reaction, which couples the reduction of INT to INT-formazan (molar extinction coefficient = 18/mM-cm at 492 nm), allowing for sensitive detection of GABAT enzyme activity in crude tissue samples. Assay solutions are stable for several years if stored and handled properly.
#GABA aminotransferase #GABAT #GABA #transamination #CNS
Kit Components:
GABAT Substrate: 2 ml, store at -80°C
TA Assay Solution: 5 ml (50 samples), store at -80°C
10x Cell Lysis Solution: 25 ml, store at 4ºC
SDS:
TX-100, DMSO, INT, Tris
Related Kits:
AST Assay, ALT Assay
Citation:
Besse et al
Personalized medicine approach confirms a milder case of ABAT deficiency
Molecular Brain 9: 93, 2016
Zhao et al
High methylation of the 4-aminobutyrate aminotransferase gene predicts a poor prognosis in patients with myelodysplastic syndrome
International J of Oncology 54: 491-504, 2019